Composite

Part:BBa_K575031

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive Promoter + RBS (B0034) + LasR

Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0034), and an RFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 904
    Illegal NheI site found at 927
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1280
    Illegal AgeI site found at 738
    Illegal AgeI site found at 850
    Illegal AgeI site found at 1477
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None